|Katarzyna Starska, Iwona Lewy-Trenda, Jan Woś, Paweł Papież, Ewa Forma, Magdalena Bryś|
Introduction. Presence of stabilized and activated form of
β-catenin in tumor tissue is connected with increased tumor
progression through activation of c-erbB-2 and c-Myc oncogene
and epithelial-mesenchymal transition (EMT).
Aim. To assess gene and protein expression of β-catenin in
the tumor tissues of laryngeal squamous cell carcinoma and to
analyze relationships between this expression on the degree of
aggressiveness of the tumor.
Material and methods. A group of 71 patients with squamous
cell carcinoma of the larynx was analyzed. The pathological
evaluation comprised pTNM classification, degree of carcinoma
invasion according to tumor front grading criteria. Quantitative
analysis of the amplified product in real time (real-time RT-PCR)
were used for estimation of mRNA β-catenin expression in
tumor cells. The level of protein expression was determined
by Western blot analysis.
Results. Increased mRNA β-catenin expression in cells of
laryngeal carcinoma was confirmed in more advanced tumors
pT3-4 (p=0.003). Tumors with higher aggressiveness identified
by the classification criteria TFG were characterized by higher
expression of β-catenin gene (p=0.002). Deep invasion and
disperse infiltration were shown to be related to higher mRNA
β-catenin expression in tumor cells (p=0.01 and p=0.003,
Conclusions. The study indicates a significant role of β-catenin
in determination of aggressiveness of tumor changes in laryngeal
squamous cell carcinoma.
keywords: rak krtani, β-kateniny, typ i głębokość inwazji
pages: from 155 to 162
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