|Krzysztof Kowal1, Joanna Osada2, Milena Dąbrowska2, Anna Bodzenta-Łukaszyk1|
1)Klinika Alergologii i Chorób Wewnętrznych, Akademia Medyczna w Białymstoku, ul. Skłodowskiej-Curie 24a, 15-276 Białystok
2)Zakład Diagnostyki Hematologicznej, Akademia Medyczna w Białymstoku, ul. Skłodowskiej-Curie 24a, 15-276 Białystok
Introduction. Recently described subpopulation of peripheral blood monocytes, which express CD16 (CD14/CD16), is characterized by increased antigen presenting ability but decreased production of anti-inflammatory mediators including interleukin-10 (IL-10). Increased percentage of CD14/CD16 cells has been found in peripheral blood of bronchial asthma patients.
Aim of study. The aim of this study was to evaluate the changes in the prevalence of CD14/CD16 cells in the peripheral blood of patients with allergic rhinitis and asthma during the course of immunotherapy (IT) with house dust mite allergen extracts.
Material and methods. The study involved on 23 patients with allergic rhinitis and mild bronchial asthma. 17 patients were treated with IT, while 6 patients received only pharmacotherapy. 10 healthy subjects served as negative controls. IT was performed using Novo Helisen Depot (Allergopharma, Germany). Blood was collected before therapy, 3 months and 12 months after the initiation of IT. Flow cytometry analysis was performed on the whole blood samples using monoclonal antibodies against CD14 and CD16 labeled with FITC and PE respectively (Pharmingen, USA).
Results. Significantly higher percentage of CD14/CD16 cells was found in the peripheral blood of patients with allergic rhinitis and asthma in comparison to healthy controls. In patients treated with IT a decrease in the percentage of CD14/CD16 cells was observed already after 3 months and the changes persisted after 12 months of IT. No significant change in the percentage of CD14/CD16 cells was observed in allergic rhinitis and asthma patients not treated with IT.
Conclusion. Changes in monocyte subpopulations during IT may represent one of the mechanisms of beneficial clinical effect of IT observed in patients suffering from allergic rhinitis and bronchial asthma.
Alergia Astma Immunologia, 2003, 8(1), 33-38
keywords: astma, immunoterapia, CD16, asthma, immunotherapy, CD16
pages: from 33 to 38
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