|Edyta Machura, Franciszek Halkiewicz, Bogdan Mazur*, Krystyna Karczewska, Ewa Goleniec|
Klinika Gastroenterologii, Alergologii i Zaburzeń Rozwoju wieku Dziecięcego Śląskiej Akademii Medycznej, ul. 3 Maja 13/15, 41-800 Zabrze
*Klinika Endokrynologii i Patofizjologii Śląskiej Akademii Medycznej, ul. 3 Maja 13/15, 41-800 Zabrze
Background. Atopic egzema dermatitis syndrome (AEDS) is a chronic inflammatory skin disorder, with the key role of immunological dysregulation. Elevated serum IgE, and eosinophilia are the major immunoregulatory abnormalities observed in patients with AEDS. Numerous studies pointed the role of activated CD4+ lymphocytes in AEDS.
Aim of study. The aim of the study was to analyse selected populations of peripheral blood lymphocytes in AEDS children.
Material and methods. The study involved 57 children (the average age was 8.5) including 16 with mild, 23 with moderate and 18 with severe AEDS. The control group consisted of 30 healthy children of the same average age. The study was performed using flow cytometry with monoclonal antibodies.
Results. A significantly higher number of CD4+ lymphocytes (p<0.02) was observed in children with AEDS. The percentage of CD3+ HLA-DR+ (p<0.05), CD25+ (p<0.001) as well as the number of CD3+ HLA-DR+ (p<0.01) and CD25+ lymphocytes (p<0.008) were increased in AEDS children.
The study revealed a positive correlation between a significantly higher severity of AEDS and the percentage (p<0.005, R:0,38), the absolute counts of CD4+ lymphocytes (p<0.01, R:0,34) and CD3+ HLA-DR+ (p<0.01, R:0.33), as well as CD4+/CD8+ (p<0.004, R:0.38). Severity of AEDS was negatively correlated with the percentage of CD8+ (p<0.006, R:-0.37), NK (p<0.01, R:-0.34). There was no significant difference in the percentage and absolute counts of CD3+, CD8+, NK and CD19 lymphocytes between AEDS and control group The expression of TCRa/b and TRCg/d was similar in both groups.
Conclusions. The results of the study confirmed the contribution of activated T lymphocytes to the pathomechanism of AEDS related to the severity of AEDS. Alergia Astma Immunologia, 2002, 7(4), 205-210
keywords: ZAZS, subpopulacje limfocytów, cytometria przepływowa, atopic egzema dermatitis syndrome, lymphocyte subpopulations, flow cytometry
pages: from 205 to 210
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