wersja polska  
Mediton - Publishing House
Start page  Journals  Books  Conferences  News  Mediton  Contact   
print this
   Allergy Asthma Immunology - archive  

Allergy Asthma Immunology
     Editorial staff
     Editorial board
     For authors
Allergology Review

 Search in articles:
  Authority of Polish Society of Allergology

vol 7. no 3. September 2002  

 Original articles
The identification of autoantibodies in patients with lupus erythematosus
Iwona Mączyńska, Elzbieta Załuga*, Stefania Giedrys-Kalemba

Katedra i Zakład Mikrobiologii i Immunologii Akademii Medycznej, ul. Powstańców Wlkp. 72, 70-110 Szczecin
*Klinika Chorób Skórnych i Wenerycznych Akademii Medycznej, ul. Powstańców Wlkp. 72, 70-110 Szczecin

Introduction. One of the laboratory criteria for diagnosis of lupus erythematosus is the presence of antibodies against nuclear antigens detected with the indirect immunofluorescence with the substrate combination Hep-2010/liver. Western blot method can be used to verify the results of Hep-2010 cells.
Aim of study. Determination of the type of autoantibodies present in the serum of patients diagnosed with lupus erythematosus using Western blot method.
Material and methods. 16 serums from patients with different forms of lupus erythematosus were subjected to analysis: SLE (9 cases), DDLE (1), SCLE (4), DLE (2). The preliminary identification of autoantibodies as well as their titer was determined on Hep-2010 cells using IIF method. Western blot test has been used to verify the results of IIF method.
Results. In patients clinically diagnosed with SLE, the following autoantibodies were identified using Western blot: RNP/Sm – 3 patients, SS-A – 2 patients, Rib.P-Prot – 1 patient. In DDLE and SCLE were identified autoantibodies RNP/Sm – 3 patients.
Conclusions. For a complete identification of autoantibodies it is necessary to use at least two diagnostic methods, for example, IIF and Western blot. Western blot method is used to verify the results of IIF method using Hep-2010 cells.
Key words: lupus erythematosus, indirect immunofluorescence (IIF), Western blot
Alergia Astma Immunologia, 2002, 7(3), 151-154

keywords: toczeń układowy, immunofluorescencja pośrednia, Western blot, lupus erythematosus, indirect immunofluorescence (IIF), Western blot

pages: from 151 to 154

estimated time of download (68 kB)
broadband speed:561282565121024[kbps]

You need to have Adobe Acrobat Reader to view the PDF file. It's free. If you don't have the Adobe Acrobat Reader plugin yet, you can get it HERE.

Start page | Journals | Books | Conferences | News | Mediton | Contact

Copyright © 1996-2020 Mediton Publishing House | All rights reserved.