|Teresa Żak-Nejmark, Maria Kraus-Filarska, Józef Małolepszy, Iwona A. Nowak, Tadeusz Dobosz*, Anna Jonkisz*, Grazyna Nadobna|
Katedra i Klinika Chorób Wewnętrznych i Alergologii AM, ul. Traugutta 57/59, 50-417 Wrocław
*Katedra i Zakład Medycyny Sądowej AM, ul. Mikulicza-Radeckiego 4, 50-368 Wrocław
Background: Mastocytes are the most important cells in an immediate allergic reaction. Following degranulation, beside the main mediator - histamine, they release several other active substances including cytokines of Th2 type. Histamine may modulate an immune response depending on the type of receptor on which it acts. Stimulating H2R on lymphocytes, histamine exerts suppressive influence on many elements of an immune response including the production of cytokines.
Aims: The aim of the study was to compare the expression of H1R and H2R mRNAs in lymphocytes of healthy subjects and pollinotic and asthmatic patients.
Methods: The study was performed on lymphocytes of 14 healthy subjects and 15 patients. RNA was isolated from peripheral blood lymphocytes . The RT - PCR reaction was performed using Titan™ One Tube RT-PCR System.
Results: The primers for histamine receptors and determined in parallel ß-actin mRNA, were labelled with fluorescent dyes, were from Bionovo Equipment and Biochemicals. Quantitative determination of the reaction products was performed by means of a capillary gel electrophoresis. The expression of H2R m RNA was higher than those for H1R in healthy subjects (the ratio H2R/H1R 1.27) whereas in patients, the expression of H2R mRNA was lower than H1R (the ratio H2R/H1R 0.86).
Conclusions: The expression of H1R mRNA did not differ significantly in both groups in contrast to the expression of H2R mRNA, which was significantly lower in atopics (p < 0.02).
Alergia Astma Immunologia, 2002, 8(2), 106-109
keywords: limfocyty, atopia, ekspresja mRNA receptorów histaminowych, lymphocytes, atopy, histamine receptor mRNA expression
pages: from 106 to 109
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