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  Authority of Polish Society of Allergology

vol 6. no 2. June 2001  

 Clinical immunology
Analysis of gene expression with microarrays - application in medicine
Rafał Pawliczak 1,, Marek L. Kowalski

Microarrays are one of the latest breakthroughs in experimental molecular biology, which allow monitoring of gene expression for tens of thousands of genes in parallel and are already producing huge amounts of valuable data. Microarray RNA expression on a genome-wide scale is now a proven technology, although the idea of analysis of expression many genes in one sample is not new. The development of clone printing technology and oligonucleotide synthesis allowed to produce high-density microarray. These systems together with more powerful and fast computer and software systems were applied not only in basic science but also in clinical medicine and pharmaceutical industry. In this publication the authors provide the information about the technology, available detection systems and data analysis software. Comprehensive review of current and fundamental papers using microarray technology application in rheumatoid arthritis, oncology, cystic fibrosis research, and allergic airways inflammation is also included.
Alergia Astma Immunologia, 2001, 6(2), 77-85

keywords: mikromacierze RNA, ekspresja genów, metody molekularne w medycynie, RNA microarrays, gene expression, molecular methods in medicine

pages: from 77 to 85

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