|Anna Maria Rogalewska, Anna Stasiak-Barmuta|
The aim of this study was to evaluate peripheral blood lymphocyte subsets in pollen-sensitive patients during specific immunotherapy (SIT). The three groups of patients were examined: group I - patients who had not received immunotherapy (n=17), group II - patients who received immunotherapy with very good or good clinical response (n=17) and group III - desensitized with doubtful results (n=8). Group II was divided into 2 subgroups - 12 persons treated for 3 years with good or very good results and 5 persons treated for 1 year with marked improvement. The majority of desensitized patients had received preseasonally a grass pollen extract- Allergovit. CD3+, CD4+, CD8+, CD19+ and NK cells were determined by flow cytometry method. The study was carried out during natural allergen exposure (June/July). In comparison with the untreated group, the percent of CD3+ cells was significantly lower (63,5±6,2 vs. 69,3±5,8%; p<0,05) and the NK cells was significantly higher (19,8±6,4 vs. 11,7±5,1%; p<0,002) in the patients treated for one year. In the group of patients desensitized for three years with good response to SIT a significantly higher percentage of NK cells was observed in comparison to the patients without SIT (18,8±6,9 vs. 11,7±5,1; p.<0,02). In patients with doubtful clinical results the percentage of CD8+ and NK cells was the lowest of all groups evaluated. Based on the results obtained it would seem that good clinical response to specific IT may be due to the cumulating of effects of suppressor/cytotoxic cells in peripheral blood. The good results of the first course of IT may predict the successful effects of subsequent immunotherapy.
Alergia Astma Immunologia, 2001, 6(1), 33-38
keywords: subpopulacje limfocytów, pyłkowica, immunoterapia swoista, lymphocyte subsets, pollinosis, specific immunotherapy
pages: from 33 to 38
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