|Mariusz Puszczewicz, Irena Zimmermann-Górska, Grażyna Białkowska-Puszczewicz |
Anti-neutrophil cytoplasmic antibodies have been shown to be a marker of Wegener's granulomatosis and vasculitis. The pathogenic role of ANCA sera is suggested. The function of neutrophils incubated with ANCA sera in comparison with autologous sera was investigated. ANCA sera were obtained from 15 patients with Wegener's granulomatosis, 6 with rheumatoid arthritis, 6 cases of polyarteritis nodosa and 3 with crescentic glomerulonephritis. ANCA serology was determinated by using a standard ELISA and indirect immunofluorescence microscopy. Phagocytosis and bactericidal capacity of neutrophils were evaluated by using fluorochrome microassay acc. to Pantazis and Kniker. The migration and chemotactic response was measured acc. to Boyden's method.
The PMNs from donors incubated with ANCA sera showed higher values of phagocytosis index than the control. No significant differences were yielded between percentage of killed staphylococci by PMNs incubated with ANCA sera, migration and chemotactic response in comparison with control ones.
These data demonstrated that ANCA sera stimulates phagocytosis of healthy donor's neutrophils. These actions may contribute to tissue damage in systemic vasculitis.
keywords: ANCA; granulocyty; guzkowe zapalenie tętnic; ziarniniak Wegenera; podostre kłębuszkowe zapalenie nerek; reumatoidalne zapalenie stawów, ANCA; neutrophils; rheumatoid arthritis; Wegener's granulomatosis; polyarteritis nodosa; crescentic
pages: from 259 to 266
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