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  Authority of Polish Society of Allergology




vol 3. no 4. December 1998  
 TABLE OF CONTENT

 Original articles
E rosetting test comparison with the fluorocytometric method in CD2+ cell evaluation
Anna Stasiak-Barmuta1, Janusz Żak2, Urszula Wiwatowska2

„^1)Zakład Alergologii Dziecięcej Akademii Medycznej, ul. Waszyngtona 17, 15-274 Białystok
2)Zakład Laboratoryjnej Diagnostyki Pediatrycznej Instytutu Diagnostyki Laboratoryjnej Akademii Medycznej w Białymstoku



The E rosetting test, recomended by WHO was considered to be the cheapest and the simplest quantitative test for counting human T lymphocytes, being very precise at the same time. In the 80's, it was found, that molecule CD2 was identical with E receptor in T cells.
The marking technique of surface cell receptors by monoclonal antibodies and their evaluation by the flow cytometry were a giant step in the development of quantitative methods assessing cell subsets. Despite its numerous advantages, this method remains to be exclusive due to very high costs of the equipment and monoclonal antibodies. The aim of this study was to evaluate the reliability of the E rosetting test in comparison with the counting CD2+ by the fluorocytometric method. 34 children aged 3-15 years were examined. We found a significant decrease in the percentage and in the absolute number of T cells evaluated by the E rosetting technique in comparison with cytometric method. There was a significant correlation between the two methods, r=0,6. In five cases a decrease number of T lymphocytes was observed simultaneously in both tests. However certain criteria increasing its objectivity should be maintained. In our opinion the E rosetting test, as it often is the only available test to be performed, could be used as a screening method for more advanced immunological diagnostics.


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