|Wiesław Szymański, Anna Maria Rogalewska, Irena Michalska, Sabina Chyrek-Borowska, Teresa Michno, Katarzyna Dechnik1|
Klinika Alergologii i Chorób Wewnętrznych Akademii Medycznej, ul. Skłodowskiej - Curie 24a, 15-276 Białystok
1)Wytwórnia Surowic i Szczepionek Biomed, ul. Sosnowa 8, 30-224 Kraków
One of the recognized methods of standardization used for determination of biological activity of a allergen is RAST inhibition test. The purpose of this study was to evaluate a usefulness of the FAST (Fluorescence Allergosorbent Test) inhibition technique in standardization of grass pollen extracts - Catalet T (Biomed). FAST inhibition test was carried out using a serum pool collected from allergic patients sensitized to grass pollen, who underwent procedure of the biological standardization before. The determination of specific IgE to Festuca elatior, Lolium perenne and Phelum pratense in the serum pool was performed according to the IgE FAST-Plus method by BioWhittaker. The value of 50% of FAST inhibition was defined with the allergen extract of grass pollen - Catalet T (IHR). The study confirmed a high level of specific IgE to evaluated allergens of Festuca elatior, Lolium perenne and Phelum pratense in the examined serum pool. The FAST inhibition test was useful in the determination of concentration of allergenic extract which caused a bound of half of specific IgE in the examined serum pool. The authors suggest that FAST inhibition test is more costly effective, easier to perform, ecologically friendly and can be used for a standardization in vitro of diagnostic and therapeutic extracts.
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