|Teresa Żak-Nejmark, Maria Kraus-Filarska, Józef Małolepszy, Renata Jankowska, Iwona Anna Nowak, Grażyna Nadobna|
Katedra i Klinika Chorób Wewnętrznych i Alergologii Akademii Medycznej, ul. Traugutta 57/59, 50-417 Wrocław
Numerous studies provide evidence that histamine is not only the main mediator of anaphylactic reactions but also a potent regulator of cellular and humoral components of the immune response. Intracellular and surface receptors on the effector cells mediate biological actions of histamine. The aim of our study was to investigate changes in histamine receptor expression to stimulation with both specific (grass pollen) and non-specific (fMLP) stimuli. The cells were obtained from healthy and allergic subjects. Lymphocytes and neutrophils were preincubated with various concentration of mixed grass pollen, Lolium perenne (1pg-1mg) and fMLP (10-8 M). Histamine fluorescein (Molecular Probes) was added and the number of cell binding histamine was determined after incubation in fluorescens microscope. Stimulation with allergens increased histamine binding significantly (p<0,01) only in allergic subjects. Stimulation with fMLP resulted in significantly (p<0,001) increased histamine binding in all subjects. We conclude that increased histamine receptor expression to both specific and unspecific stimulation appears to be associated with lymphocytes and neutrophils activation.
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