|Izabela Kupryś, Grażyna Józefowicz, Piotr Kuna|
Klinika Pneumonologii i Alergologii Instytutu Medycyny Wewnętrznej Akademii Medycznej, ul. Kopcińskiego 22, 90-153 Łódź
Although heparin's anticoagulant properties dominate its use in medicine, there is more and more evidence of its beneficial effect in allergic diseases. However, the mechanism underlying the antiallergic activity of heparin is still little known. Because IgE synthesis by lymphocytes B is the first step in the development of allergic response, the goal of our experiment was to investigate the effect of heparin on IgE production.
The experiment was performed on peripheral blood mononuclear cells (MNCs) isolated from atopic donors. The cells were incubated for 10 days in the absence or presence of heparin, used in different concentrations (10, 100, 1000 U/ml). We investigated its influence on spontaneous and interleukin 4 (10 ng/ml)- or lipopolisaccharide (10 ng/ml)-stimulated IgE synthesis. After culture and evaluating cell viability the supernatants were assayed for IgE level by ELISA test and the sediments for percentage of blasts using microscopic preparation stained with May-Grunwald-Giemsa method.
The results showed that heparin in dose 10 IU/ml significantly decreases IgE synthesis in the spontaneous cultures (p<0.034). This effect was not observed in other experiments. Heparin did not also alter, in any significant way, cell proliferative responses or cell viability. Such results do not prove that heparin inhibits IgE synthesis. We concluded that lack of heparin supressory effect in our experiment might be caused by firstly - neutralisation of heparin by platelet factor 4 released from platelets present in our cultures, secondly - applied doses of heparin because the activity of heparin is dose-dependent.
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