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Allergology Review

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  Authority of Polish Society of Allergology

vol 20. no 4. December 2015  

 Original articles
Parainfluenza type 3 (PIV-3) infection of primary bronchial epithelial cells grown in vitro as an experimental model of bronchial asthma exacerbations
Sylwia Moskwa, Wojciech Piotrowski, Jerzy Marczak, Anna Lewandowska-Polak, Marzanna Jarzębska, Małgorzata Brauncajs, Anna Głobińska, Aleksandra Piechota-Polańczyk, Paweł Górski, Marek L. Kowalski

Introduction. Model of primary bronchial epithelial cells culture has
been used to study the innate response to viral infections in patients with
bronchial asthma.
Aim. The aim of the study was to evaluate the effectiveness of bronchial
epithelial cell culture and the influence of parainfluenza virus type 3 (PIV-
3) on cell morphology.
Materials and methods. Human Bronchial Epithelial cells (HBECs) were
obtained during bronchoscopy from 31 subjects: 12 healthy subjects and
19 patients with bronchial asthma. The cells were grown in BEGM culture
medium (Bronchial Epithelial Cell Growth Medium) and were infected
with PIV-3 0.1 MOI.
Results. Successful cultures were obtained for 61.3% of HBECs. Fungal
infection was the main reason for the failure to grow HBECs. No cytopathic
effect of PIV3 on epithelial cells was observed for up to 48 hours
after infection.
Conclusions. The described methodology enables successful culture of
primary bronchial epithelial cells and their infection with PIV3.

keywords: zaostrzenia astmy, infekcje wirusowe, komórki nabłonka,asthma exacerbations, viral infections, epithelial cells

pages: from 264 to 268

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