|Sylwia Moskwa, Wojciech Piotrowski, Jerzy Marczak, Anna Lewandowska-Polak, Marzanna Jarzębska, Małgorzata Brauncajs, Anna Głobińska, Aleksandra Piechota-Polańczyk, Paweł Górski, Marek L. Kowalski|
Introduction. Model of primary bronchial epithelial cells culture has
been used to study the innate response to viral infections in patients with
Aim. The aim of the study was to evaluate the effectiveness of bronchial
epithelial cell culture and the influence of parainfluenza virus type 3 (PIV-
3) on cell morphology.
Materials and methods. Human Bronchial Epithelial cells (HBECs) were
obtained during bronchoscopy from 31 subjects: 12 healthy subjects and
19 patients with bronchial asthma. The cells were grown in BEGM culture
medium (Bronchial Epithelial Cell Growth Medium) and were infected
with PIV-3 0.1 MOI.
Results. Successful cultures were obtained for 61.3% of HBECs. Fungal
infection was the main reason for the failure to grow HBECs. No cytopathic
effect of PIV3 on epithelial cells was observed for up to 48 hours
Conclusions. The described methodology enables successful culture of
primary bronchial epithelial cells and their infection with PIV3.
keywords: zaostrzenia astmy, infekcje wirusowe, komórki nabłonka,asthma exacerbations, viral infections, epithelial cells
pages: from 264 to 268
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