|Magdalena Kujawiak, Zuzanna Lech, Joanna Makowska, Barbara Bieńkiewicz, Marek L. Kowalski, Janina Grzegorczyk|
Background. Drug-induced hypersensitivity reactions constitute
approx. 20-25% of all adverse drug reactions. Hypersensitivity to
non-steroidal anti-inflammatory drugs (NSAIDs) occurs in about
25% of the cases. The diagnosis is based only on medical history of
a patient and provocation test. In vitro tests are alternative diagnostic
Aim. The aim of the study was to evaluate the effect of aspirin and
other NSAIDs on CD63 expression on basophils, and on histamine
release from peripheral blood basophils in patients with aspirin-induced
urticaria (AIU) as compared to healthy individuals.
Material and methods. Twenty patients with the history of AIU and
10 healthy aspirin-tolerant individuals (HAT) were recruited. CD63
expression was analyzed by flow cytometry. Histamine release was
measured by enzyme-linked immunosorbent assay.
Results. Sensitivity for basophil activation test amounted to 35,5%,
45% and 45% for 1μM, 20μM and 200μM concentrations of aspirin
respectively. Specificity amounted to 90%, 80% and 60% respectively.
For all concentrations tested, except 200μM ASA which activated
basophils in both AIU patients and healthy individuals, considering
response to at least one concentration of aspirin, sensitivity amounted
to 45% and specificity 80%. Sensitivity for histamine release test
amounted to 60% and specificity to 66,5%. There was no correlation
between histamine release and CD63 expression on basophils.
Conclusions. Our results suggest a relatively low diagnostic value of
both tests examined. Basophil activation test seems to be of higher
value than of histamine release test.
keywords: drug hypersensitivity, aspirin, basophil activation test, (BAT), histamine, urticaria
pages: from 214 to 220
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