|RYSZARD RUTKOWSKI, ANETTA TATARCZUK-KRAWIEL, TADEUSZ MONIUSZKO, KRZYSZTOF RUTKOWSKI, ŁUKASZ STACHURSKI, MARTA BILBIN|
Introduction. Chronic inflammatory process in non-atopic asthma suggests impaired anti-inflammatory mechanisms with a probable crucial role of IL-10.
Aim of the study. Evaluate the effect of rhIL-10 on IL-1?, IL-6 and TNF-? production by peripheral blood monocytes in LPS-stimulated cultures.
Material and methods. The study was performed on 32 patients with mild non-atopic asthma and a control group of 20 healthy blood donors. IL-1?, IL-6, TNF-? concentrations in cell culture supernatants were assessed by immunoenzyme assay.
Results. Peripheral blood monocytes from non-atopic asthmatics compared to controls release greater amount of IL-1? spontaneously as well as after stimulation with LPS, while more IL-6 and TNF-? is released only in the endotoxin-stimulated cultures. In asthmatic patients, monocyte IL-1? and TNF-? production was inhibited by rhIL-10 to a lesser extent than in controls. No such effect was observed for IL-6.
Conclusions. Our results suggest increased monocyte activity in non-atopic asthmatics, possible stimulating role of endogenous factors and these cells' altered sensitivity to inhibitory effects of IL-10 on the production of some proinflammatory cytokines.
keywords: rhIL-10, cytokiny prozapalne, monocyty, nieatopowa astma oskrzelowa, rhIL-10, proinflammatory cytokines, monocytes, non-atopic asthma
pages: from 40 to 45
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