|Patrycja Zawadzka, Mirosław Szczepański, Liliana Łykowska-Szuber, Husam Samara, Krzysztof Linke, Jan Żeromski|
Introduction. Inflammatory bowel diseases are chronic diseases of gastrointestinal tract which include ulcerative colitis and Crohn's disease. About 5 to 15% of the cases are difficult to diagnose using conventional clinical, radiological, endoscopic and microscopic criteria. Antibodies pANCA and GAB are serologic markers of ulcerative colitis, while antibodies ASCA and PAB are characteristic for Crohn's disease.
Aim of the study. Evaluate the role of pANCA, GAB, ASCA and PAB in diagnosing and identifying inflammatory bowel diseases.
Material and methods. Serum samples were obtained from 20 patients with diagnosed Crohn's disease, 20 patients with ulcerative colitis and 20 healthy controls. Autoantibodies were assessed using the indirect immunofluorescence technique.
Results. In ulcerative colitis, pANCA were present in 65% and GAB in 25% patients. In Crohn's disease, ASCA were present in 65% and PAB in 50% subjects. In ulcerative colitis the sensitivity and specificity of pANCA was 65 and 95% and that of GAB was 25% and 95% respectively. In Crohn's disease, the sensitivity and specificity of ASCA was 68% and 95% and that of PAB was 47% and 95%, respectively.
Conclusions. Assessment of pANCA, GAB, ASCA and PAB together can be used as a serologic panel for diagnosing and identifying inflammatory bowel diseases.
keywords: wrzodziejące zapalenie jelita grubego, choroba Leśniowskiego-Crohna, przeciwciała pANCA, ASCA, GAB, PAB, ulcerative colitis, Crohn's disease, autoatibodies pANCA, GAB, ASCA, PAB
pages: from 155 to 161
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