|Edyta Machura, Bogdan Mazur, Augustyn Folwaczny, Krystyna Karczewska, Ewa Golemiec, Jolanta Kaufman|
Introduction. Atopic eczema (AE) is a chronic inflammatory skin disorder which often leads to asthma or allergic rhinitis. Although Th2 cytokines are recognised to play a key role in the pathogenesis of AE, also INF-? contributes to chronic skin lesions. It is known that the extent of S.aureus skin colonisation correlates with AE disease activity and contributes to changes in the immunological profile.
Aim of the study. Determine the percentage of peripheral blood CD3+, CD4+, CD8+, CD3+CD25+, CD4+CD25+ T cells as well as intracellular production of IL-2, IL-4, INF-? and TNF-? by peripheral blood CD3+T cells from AE children with respect to the degree of S.aureus skin colonisation.
Material and methods. The study involved 15 children, including 6 (average age 11,2±1,7.) with S.aureus skin colonization (S.aureus+), and 9 (average age 12,3±0,97) without bacteria on skin (S.aureus-). The control group consisted of 7 healthy children with the same age average, i.e. 11,5±1,3. T cell subsets and intracellular cytokine production after p-methoxyamphetamine (PMA)+ionomycin stimulation were measured by flow cytometry.
Results. There was no difference in lymphocyte subsets and cytokine expression between AE children without bacteria on the skin (S.aureus-) and healthy controls. There was a significant decrease in the percentage of CD4+ (p<0,01) and an increase in the percentage of CD3+ T cells producing TNF-? (p<0,03) upon in vitro stimulation with PMA and ionomycin in children with skin colonization (S.aureus+). The duration of AE was inversely correlated both with absolute number of CD3+ (R:-0,58, p<0,02), and the percentage of CD3+ T cells producing INF-? (R:-0,67; p< 0,006) and IL-2 (R:-0,75; p<0,001).
Conclusions. A decrease in the percentage of CD4+ T cells and increased intracellular production of TNF-? by peripheral blood CD3+ T cells in a subgroup of children with AE may result from AE and the coexisting Staphylococcus aureus skin infection.
keywords: WA, populacje limfocytów, cytokiny, AE, lymphocyte subsets, cytokines
pages: from 42 to 48
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